Mechanisms of action of 5-bromodeoxyuridine based on studies with tetrahymena pyriformis
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منابع مشابه
The Effect of 5-bromodeoxyuridine on Dna Replication and Cell Division in Tetrahymena Pyriformis
Populations of Tetrahymena pyriformis were grown in a chemically defined medium containing the thymidine analogue 5-bromodeoxyuridine (BUdR). About 65% of the thymidine sites in DNA were substituted by BUdR. During the first generation in the presence of BUdR, all DNA became hybrid. After the following cell division, in about 80% of the cells the second DNA replication round was initiated but n...
متن کاملInhibition of rRNA synthesis following incorporation of 5-bromodeoxyuridine into DNA of Tetrahymena pyriformis.
Tetrahymena pyriformis was grown on chemically defined medium in the presence of 5-bromodeoxyuridine (BUdR). Under these growth conditions more than 60% of the thymidine sites in DNA were substituted with BUdR. It was found that RNA synthesis was strongly inhibited by the presence of BUdR in DNA. To assure that incorporation of BUdR into DNA was a prerequisite of the effect observed, BUdR was a...
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A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
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Since the discovery (1, 2) and elucidation of the structure of 5-ribosyluracil ($ uridine) (3-5)) increasing attention has been turned toward the metabolism of this cotnpound. $ Uridine, along with the other new]> discovered constituents of ribonucleic acid, has been shown to be present in highest proportions, if not exclusively, in soluble, or amino acid transfer RX.1 (2, 6). Because of the ce...
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Meyerhof, 0. (1945). J. biol. Chem. 157, 105. Meyerhof, 0. & Kaplan, A. (1951). Arch. Biochem. Biophy8. 38, 282. Meyerhof, 0. & Oesper, P. (1947). J. biol. Chem. 170, 1. Meyerhof, 0. & Wilson, J. R. (1949). J. biol. Chem. 180, 575. Nelson, N. (1944). J. biol. Chem. 153, 373. Nilsson, R. & Alm, F. (1941). Biochem. Z. 308, 262. Prankerd, T. A. J. & Altman, K. I. (1954). Biochem. J. 58, 622. Roe, ...
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ژورنال
عنوان ژورنال: Carlsberg Research Communications
سال: 1977
ISSN: 0105-1938
DOI: 10.1007/bf02906709